The short answer is No. Whether you are using the software on the Free Trial time period or you have actually purchased the software, there is NO difference in functionality or usability. The only difference is that the Free Trial period will only allow you to use the software for the time allowed.
There is a 20-Day Free Trial included for the AxMaster software
Once you purchase the software on the Fretlight Store, you will be e-mailed a registration key to unlock the software in 2-4 business days.